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Kinetic parameters for hydrolysis of NPPC by pneumococcal Pce in buffer A at different Zn2+ concentrations
| [Zn2+] (μM) | Km (mM) | kcat (s−1) | kcat/Km (mM−1 s−1) |
| 3 | 0.17 ± 0.02 | 9.8 ± 0.2 | 57 ± 6 |
| 200 | 0.9 ± 0.1 | 10.5 ± 0.2 | 12 ± 1 |
| 400 | 1.2 ± 0.2 | 10.0 ± 0.3 | 8.3 ± 0.6 |
| 800 | 2.2 ± 0.4 | 9.9 ± 0.2 | 4.5 ± 0.2 |
Measurements were carried out at 25°C (I = 0.05).
