Table 6.
Protein | 3 μM Zn2+ | 0.5 mM Zn2+ | 1 mMZn2+ | 2 mM Zn2+ |
Wild type | 796 | 68 | 11 | <1 |
H90A | 66 | 7 | <1 | <1 |
Measurements were made at 25°C in buffer A. Activity is expressed in units per mg. One unit corresponds to 700 cpm of [H3-methyl] choline released in 10 min. Estimated errors are around 5%.