Figure 1.
Gel of immunoaffinity copurified proteins. Antibody against MT3 was immobilized on Affigel-Hz and used to isolated MT3 and associated proteins. Proteins were applied and eluted as described and analyzed by SDS-gel electrophoresis and mass spectrometry. Proteins identified by mass spectrometric analysis are labeled. Lane 1 is the recovered protein after elution with NaSCN. Lane 2 shows the proteins in the nonretained pool after incubation of whole-brain extract with the immobilized antibody. Lane 3 shows the positions of the prestained marker proteins with values of Mr shown.