Figure 6.

The all-to-all pairwise analysis of sequence window phylogenetic similarity is plotted. Lighter colors indicate higher similarity; darker colors indicate less. The identified phylogenetic motifs are numbered. Additionally, all strongly (greater than ±0.5 kcal/mol) interacting pairwise electrostatic interactions are laid on top of the phylogenetic comparisons. Stabilizing interactions are colored white; destabilizing interactions are gray. Many of the catalytic electrostatic network interactions are predicted by the phylogenetic comparisons. On the other hand, there are many electrostatic interactions, largely involved in stabilization of the structure, that are not predicted. Circles indicate the four phylogenetic motifs strongly interacting with Glu169 (see Fig. 5A ▶).