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Thermodynamic parameters for binding of MUP-IV to pheromone analogs
| Ligand | Kd (μM) | Stoichiometry (n) | ΔH (kcal/mol) | −TΔS (kcal/mol) | ΔG (kcal/mol) | Kd(analog)/Kd(MT) |
| MT | 0.2 ± 0.01 | 1.0 ± 0.1 | −16.7 ± 0.1 | +7.4 ± 0.1 | −9.3 ± 0.1 | 1 |
| MP | 1.0 ± 0.1 | 1.0 ± 0.1 | −11.6 ± 0.1 | +3.2 ± 0.1 | −8.4 ± 0.1 | 5.0 |
| MO | 25 ± 3 | 3.4 ± 0.1 | −5.1 ± 0.2 | −1.3 ± 0.2 | −6.4 ± 0.1 | 125 |
| MF | (no detectable signal) | |||||
| MCP | (weakly exothermic signal) | |||||
All values were obtained by fitting ITC data at 30°C, as described in Materials and Methods.
