Table 2.
Masses of modified proteins and peptide fragments
| Polypeptide | Theoretical mass (Da)a | Experimental mass (Da)b |
| MBPwt::ZifQNK | 46,213 | 46,200 |
| MBP141(TMR)::th::ZifQNK(Cy5)2 | 48,250 | 48,317 |
| MBP141(TMR)c | 41,820 | 41,873 |
| ZifQNK(Cy5)c | 5657 | 5663 |
| ZifQNK(Cy5)2c | 6435 | 6446 |
| MBP141(Cy5)::th::ZifQNK(TMR)2 | 47,953 | 47,814 |
| MBP141(Cy5)c | 42,117 | 42,157 |
| ZifQNK(TMR)c | 5360 | 5386 |
| ZifQNK(TMR)2c | 5842 | 5866 |
| βZif(IAF)2::th::MBP141(TMR)::th::ZifQNK(Cy5)2 | 51,417 | 51,578 |
a Theoretical masses calculated using DNA Strider version 1.2.
b Experimental masses measured using MALDI-TOF mass spectrometer, as described in Materials and Methods.
c Peptide fragments obtained by thrombin cleavage.