Figure 2.

Ribbon representation of the structures of J domains from various Hsp40 and Hsp40-like proteins. The structures for E. coli DnaJ (1XBL, 1BQZ, 1BQ0) (Pellecchia et al. 1996; Huang et al. 1998), human HDJ1 (1HDJ) (Qian et al. 1996), and the murine polyomavirus T antigen (1FAF) (Berjanskii et al. 2000) J domains were determined using NMR. The structure of E. coli Hsc20 (1FPO) (Cupp-Vickery and Vickery 2000), SV40 T antigen (1GH6) (Kim et al. 2001), and bovine auxilin (1NZ6) (Jiang et al. 2003) J domains were determined using X-ray crystallography. The structures were visualized using Molscript (Kraulis 1991). The HPD motif is depicted in purple. Helices II and III are in blue, with the more mobile helices I and IV in red. The helices and HPD motif are labeled on the DnaJ 1XBL J domain structure. Only the J domain region is shown even if additional structural regions were determined.