Table 1.

Summary of J domain swapping experiments

J domain source		C terminus source
Proteina	Type	Protein	Type	Function	Method	Reference
Sis1	II	Sec63	III	No	Complementation assays in yeast cells not producing Sec63	Schlenstedt et al. 1995
Scj1	I			Yes
Mdj1	I			No
Mdj1	I	DnaJ	I	Yes	Complementation for lack of E. coli DnaJ and CbpA	Deloche et al. 1997
DjlA	III	DnaJ	I	Yes	Complementation for lack of E. coli DnaJ and CbpA	Genevaux et al. 2001
DjlC	III	DnaJ	I	No	Inability to replace E. coli DnaJ in vivo	Kluck et al. 2002
Large T Agb	III	DnaJ	I	Yes	Complementation for lack of E. coli DnaJ and CbpA	Kelley and Georgopoulos 1997
		Ydj1	I	Yes
DnaJ	I	Large T Ag	III	No	Inability to form infectious virions; ineffective DNA replication; inability to stimulate ATPase activity	Sullivan et al. 2000b
Ydj1	I			No
Hdj1	I	Large T Ag	III	Yes	Mediating reduction in levels of phosphorylated p130 protein	Stubdal et al. 1997
Hsj1	II			Yes
P58IPK	III	DnaJ	I	Yes	Complementation for lack of E. coli DnaJ and CbpA; substitute for lack of Ydj1 in yeast	Yan et al. 2002
		Ydj1	I	Yes
Hsj1	II	Large T Ag	III	Yes	Allows efficient viral DNA replication	Campbell et al. 1997
DnaJA2	I			Yes
Hdj1	I	DnaJ	I	Yes	Complementation for lack of E.coli DnaJ and CbpA	Genevaux et al. 2002
Hsj1	II	T antigen	III	Yes	Functional in T Ag mediated cellular transformation	Zalvide et al. 1998
T Ag	III	Ydj1	I	Yes	Substitutes for lack of Ydj1 in yeast	Fewell et al. 2002

^a Sis1, Scj1, Mdj1, and Ydj1 are from Saccharomyces cerevisiae; DnaJ, DjlA, and DjlC are from E. coli; Hdj1, Hsj1, DnaJA2, and P58^IPK are from Homo sapiens.

^bThe large T antigen J domains from SV40, JC virus, and BK virus were used in this experiment.