Table 2.
Effect of substitutions performed in the J domain
| Mutation | Helix | Protein | Effect | Reference |
| L13M; L13I; L14V | I | T Ag | Functional | Li et al. 2001 |
| L13V | I | T Ag | Decrease in viral DNA synthesis | Li et al. 2001 |
| L17K | I | Large T antigen | Nonfunctional—reduction of levels of p130 | Stubdal et al. 1997 |
| Y7A; L10A | I | A. tumefaciens DnaJ | Lack of complementation | Hennessy et al. 2005 |
| A33R; Y34K | II | Large T antigen | Functional—reduction of levels of p130 | Stubdal et al. 1997 |
| RE(19,20)AA; RK(22,23)AA; R27A; L28A; A29G; A29G; MK(30,31)AA; Y32A | II | E. coli DnaJ | No effect | Genevaux et al. 2002 |
| E20A; K22A; K27A; Y32A | II | A. tumefaciens DnaJ | No effect | Hennessy et al. 2005 |
| RK(22,23)AA/KR(26,27)AA; Y25A, K26A; KR(26,27)AA; K26E | II | E. coli DnaJ | Lack of complementation | Genevaux et al. 2002 |
| R26A; RK(26,27)AA | II | A. tumefaciens DnaJ | Lack of complementation | Hennessy et al. 2005 |
| W24R; L29P; M30T; Y34N; A37V; C38R | II | T Ag-Ydj1 chimera | Lack of complementation | Fewell et al. 2002 |
| Y24A; RR(25,26)AA | II | Hdj1-DnaJ chimera | Lack of complementation | Genevaux et al. 2002 |
| YKR(25-27)AAA | II | E. coli DnaJ | Toxic | Genevaux et al. 2002 |
| A181T | III | Sec63 | Translocation defects | Lyman and Schekman 1995 |
| F45A | III | Hdj1-DnaJ chimera | Lack of complementation | Genevaux et al. 2002 |
| F46L | III | Ydj1 | Lack of complementation | Johnson and Craig 2000 |
| F47A | III | E. coli DnaJ | Lack of complementation | Genevaux et al. 2002 |
| K51E; K53R; M55T; N56D; Y59N | III | T Ag-Ydj1 chimera | Lack of complementation | Fewell et al. 2002 |
| KE(41,42)AA; E44A; K46A; KE(48,49)AA; KE(51,52)AA; Y54A; E55A; T58A; TD(58-59)AA | III | E. coli DnaJ | No effect | Genevaux et al. 2002 |
| K46T; F47L; K48T; A53S | III | A. tumefaciens DnaJ | No effect | Hennessy et al. 2005 |
| L57S; D59A | IV | A. tumefaciens DnaJ | Lack of complementation | Hennessy et al. 2005 |
| K62A; R63A; K62A,R63A | IV | E. coli DnaJ | Diminished interaction with DnaK by SPR | Laufen et al. 1999 |
| R63A | IV | A. tumefaciens DnaJ | Variable ability to complement | Hennessy et al. 2005 |
| SQ(60,61)AA; KR(62,63)AA; DQ(66,67)AA | IV | E. coli DnaJ | No effect | Suh et al. 1999 |
| D59N; K62A; Y66A; D67A; D67N | IV | A. tumefaciens DnaJ | No effect | Hennessy et al. 2005 |
| D35N | Loop | E. coli DnaJ J domain | Diminished interaction with DnaK | Wittung-Stafshede et al. 2003 |
| D44N | Loop | T Ag | Nonfunctional | Sullivan et al. 2000a |
| H233Q; D235N | Loop | DjlA-DnaJ chimera | Lack of complementation | Genevaux et al. 2001 |
| H32Q | Loop | Hsp40 | Inhibition of Hsp70 luciferase refolding | Michels et al. 1999 |
| H33Q | Loop | Ydj1 | No stimulation of ATPase | Tsai and Douglas 1996 |
| H33Q | Loop | E. coli DnaJ | Lack of complementation | Kelley and Georgopoulos 1997 |
| H33Q | Loop | E. coli DnaJ | Lack of interaction with DnaK by SPR | Mayer et al. 1999 |
| H33Q | Loop | E. coli DnaJ | Stimulation of DnaK lost | Laufen et al. 1999 |
| H33Q; ΔH33; ΔP34; P34F; ΔD35 | Loop | E. coli DnaJ | Lack of complementation | Genevaux et al. 2002 |
| H42Q; D44N | Loop | T Ag | Nonfunctional | Zalvide et al. 1998 |
| H42Q; D44N | Loop | Large T antigen | Nonfunctional—reduction of levels of p130 | Stubdal et al. 1997 |
| H42R; D48G | Loop | T Ag-Ydj1 chimera | Lack of complementation | Fewell et al. 2002 |
| H43Q; D45A | Loop | Csp | No stimulation of Hsc70 ATPase | Chamberlain and Burgoyne 1997 |
| H89Q | Loop | Mtj1 J domain | No stimulation of BiP ATPase: Poor interaction | Chevalier et al. 2000 |
| HPD-AAA | Loop | P58IPK | No stimulation of Hsc70 ATPase | Yan et al. 2002 |
| K35G | Loop | Hdj1-DnaJ chimera | Marked effects on function | Genevaux et al. 2002 |
| N36G | Loop | Hdj1-DnaJ chimera | No effect | Genevaux et al. 2002 |
| R36G; N37G; Q38G | Loop | E. coli DnaJ | Partial complementation | Genevaux et al. 2002 |
| RNQ (36-38) GGG | Loop | E. coli DnaJ | Lack of complementation | Genevaux et al. 2002 |
| H33Q; HD(33,35)YY; D35E | Loop | A. tumefaciens DnaJ | Lack of complementation | Hennessy et al. 2005 |