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Dependence of the protonation rate constant (k) of EGFP chromophore on solvent viscosity (η) (open circles). k and k0 are the protonation rate constants of EGFP at solvent viscosities of η and η0, respectively. k0 and η0 correspond to the situation in the absence of viscogen (glycerol). Continuous line shows the least square fit with slope=1.09 and the dotted line corresponds to Kramers’ relationship (slope=1) between the rate constant and the solvent viscosity (k∝1/η).
