Table 3.

Estimation of secondary structure content in the fragments

Fragment	Helix (% content)	β (% content)	Turn (% content)	Random (% content)
m53BP1[1463–1617]	6 ± 3	74 ± 0	0	19 ± 3
Stag m53BP1 [1466–1535]	12.4 ± 2	45 ± 5	14 ± 2	28.6 ± 3
yRad9[754–947]	0 ± 0	73 ± 3	0 ± 0	27 ± 3
Stag yRad9[754–947]	0 ± 0	69 ± 1	6 ± 0	25 ± 1
yRad9[764–947]	N.D.	N.D.	N.D.	N.D.
Stag yRad9[764–855]	22 ± 0	22 ± 7	24 ± 7	33 ± 0

The values were obtained by analyzing the CD spectra shown in Figure 3B ▶ according to Dr. Yang’s program (reference method of JASCO 810 apparatus). Values are given as the mean (± SD) of two experiments. (N.D.) Nondetermined.