Table 2.
Analysis of RNA
| Culture conditions | |||||
| RNA analyzed | 30°C pH 5 | 30°C pH 7 | 20°C pH 5 | 35°C pH 5 | 35°C pH 7 |
| FPS1-ectopic | |||||
| Glucose phase | 47 (16) | 941 (208) | 136 (40) | 103 (21) | 435 (64) |
| Ethanol phase | 55 (15) | 535 (69) | 115 (30) | 140 (35) | 174 (73) |
| FPS1-endogenous | |||||
| Glucose phase | 11 (1) | 81 (5) | N.D. | 10 (1) | 19 (3) |
| Ethanol phase | 13 (1) | 45 (6) | N.D. | 12 (1) | 11 (3) |
| TPI1 | |||||
| Glucose phase | 41 (12) | 76 (23) | 45 (3) | 23 (6) | 68 (6) |
| Ethanol phase | 25 (1) | 35 (9) | 21 (3) | 21 (4) | 18 (5) |
| IPP1 | |||||
| Glucose phase | 7 (1) | 4 (1) | 7 (1) | 6 (1) | 6 (1) |
| Ethanol phase | 4 (1) | 3 (1) | 4 (1) | 6 (1) | 5 (1) |
| ACT1 | |||||
| Glucose phase | 78 (11) | 83 (16) | 85 (9) | 68 (5) | 94 (4) |
| Ethanol phase | 71 (5) | 57 (10) | 91 (9) | 76 (8) | 68 (12) |
| PDA1 | |||||
| Glucose phase | 5 (1) | 6 (1) | 4 (0) | 5 (0) | 3 (0) |
| Ethanol phase | 5 (0) | 6 (1) | 4 (0) | 5 (1) | 3 (0) |
Analysis of mRNA was performed using real time Q-PCR, which yielded copies of mRNA/cell. The data were normalized using the reference genes IPP1, PDA1, ACT1, and the signal was scaled to mRNA copies/cell according to a SAGE study (Velculescu et al. 1997), in which copies of mRNA/cell of all the reference genes had previously been determined. Standard error of the mean, in parentheses, was calculated for the samples taken in the glucose (n=3; points 1, 2, 3) and ethanol (n=3 or 4; points 5, 6, 7, [8]) growth phases.