Figure 4.
Tetramer–dimer dissociation constants at pH 7.5. The gel filtration procedure used to determine these tetramer–dimer dissociation constants (Kd values) and the treatment of the data have been described in detail in Manning et al. (1996, 1999). The plots show the amounts of Hb present on the column during the analysis to attain a certain percent of tetramer, the functional O2-carrying structure of Hb. Further treatment of the data is shown in the inset where the Kd value can be read directly as the intersection point of the horizontal line at 1 on the Y axis with the experimental line.