Table 3.

Thermodynamic binding parameters for representative SH2 domains

SH2 domain	Peptide	ΔHa	−TΔSa	KD (μM)	Techniqueb	Reference
Grb2	Shc	−7.9	−1.2	0.20	ITC	McNemar et al. 1997
Grb2	erbB1	N/A	N/A	0.004	SPR	Suenaga et al. 2003
	erbB4	N/A	N/A	0.19	SPR
Grb2	Shc	N/A	N/A	0.38	SPR	deMol et al. 2004
Src	pYEEI	−8.4	−0.12	0.55	ITC	Ladbury et al. 1995
Src	pYEEI	N/A	N/A	0.67	SPR	Ladbury et al. 1995
Src	pYEEI	−7.3		0.60	ITC	Bradshaw and Waksman 1998
Src	pYEEI	−9.3	−0.33	0.10	ITC	Chung et al. 1998
Lck	EGFR (erbB1)	−1.1 to −1.5		7.1 to 3.3	ITC	Lemmon and Ladbury 1994
Lck	Lck(pY505)	−8.4	1.1	4.2	ITC	Ladbury et al. 1995
p85α N-term	PDGFR	−9.4	−0.73	0.47	ITC	Ladbury et al. 1995
p85α N-term	PDGFR	N/A	N/A	0.24	SPR	Ladbury et al. 1995
p85α C-term	PDGFR	−11.2	1.3	0.048	ITC	O’Brien et al. 2000
p85α C-term	PDGFR	N/A	N/A	0.064	SPR	Panayotou et al. 1993

^a Kcal/mol.

^b ITC, isothermal titration calorimetry; SPR, surface plasmon resonance.