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Effect of an R9 tag on the purification of a protein by cation-exchange chromatography. (A) RNase A-R9 was purified by cation-exchange chromatography before (−CPB) and after (+CPB) the addition of carboxypeptidase B. (B) SDS-PAGE gel of RNase A-R9 before (−CPB) and after (+CPB) the addition of carboxypeptidase B. Purified RNase A is a standard.
