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Effect of an R9 tag on the conformational stability of a protein. (Top) Thermal denaturation of RNase A (□) (Tm=63.7 ± 1.0°C) and RNase A-R9 (▪) (Tm=54.0 ± 1.0°C) in PBS. (Bottom) Conformational stability of RNase A (□) and RNase A-R9 (▪) in 50 mM sodium phosphate buffer (pH 7.2), containing NaCl (0–1.00 M).
