Figure 5.

(Left panel) Cross-linking of p25α, lysozyme, and α-synuclein with BS3 at different urea concentrations. BS3 cross-linking leads to the formation of a second faster-migrating band in the case of p25α and lysozyme (indicated by arrows), which for p25α disappears at higher urea concentrations. (Right panel) Digestion of p25α and lysozyme in the presence of various concentrations of protease. (A) p25α in PBS buffer with trypsin. (B) p25α in 0.5 M Na₂SO₄ with trypsin. (C) p25α in PBS buffer with chymotrypsin. (D) Lysozyme in PBS buffer with trypsin. The numbers on the gel indicate the fraction of protease relative to p25α or lysozyme (w/w). Note that the digestion pattern for p25α is the same when we stabilize the native state (in 0.5 M Na₂SO₄).