Table 1.

Quantification and activity assay of purified proteins

	Purified protein concentration
Target protein	mg/liter culture	mg/g dry cell weighta	Activity
MBP	36.3 ± 2.2	3.35 ±0.2	Affinity to Maltose resin
β-Galactosidase	39.6	3.67	91.0 units/mg purified lysatec
CAT	86.0b	7.96	N/A
NusA	34.3 ±2.8	3.17 ±0.26	N/A

^a Approximate cell pellet weight for 1-mL culture: 27.0 mg. Approximate dry cell weight: 10.8 mg.

^b This protein content includes the impurities (PPI) shown in lane 9 of Fig. 5B ▶.

^cUnit definition: One unit will hydrolyze 1.0 μmol of o-nitrophenyl β-D-galactoside (ONPG) to o-nitrophenol and D-galactose per minute.