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SDS-PAGE analysis of crmA-elastase reactions. Elastase (5 μM) was incubated with wild-type (lanes 3,4), P1 Arg (lanes 6,7), and P1 Gly-P1′Arg (lanes 9,10) crmAs (5 μM) for 5 min and 30 min and reactions quenched with SDS sample buffer before heat denaturation and electrophoresis. Elastase alone (lane 1) and wild-type (lane 2), P1 Arg (lane 5), and P1 Gly-P1′ Arg (lane 8) crmAs alone.
