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Negative stained electron micrographs of purified wild-type and mutant NωV VLPs at pH 7.6 and pH 5.0. E103Q (g,h) and E103S (e, f ) formed aberrant assembly products. E103D (c,d) and T246S (i, j ) formed particles that resembled wild type (a,b). No particles were detected for T246A, K521A, and K521R. Arrows in (e) and ( f ) indicate E103S mutant particles that resemble wild-type particles in size and appearance. Scale bar is 200 nm.
