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. 2005 Apr;14(4):902–913. doi: 10.1110/ps.041048805

Figure 4.

Figure 4.

Kinetics of the conversion of MER-45-kDa hGH into 22-kDa hGH in the absence and presence of divalent metal cation chelators under nonreducing conditions. (Section I) Analytical SDS-PAGE pattern of the dissociation of the MER-45-kDa hGH at 95°C in the absence of EDTA/EGTA (A), in the presence of 1 mM EDTA/EGTA (B), in the presence of 10 mM EDTA/EGTA (C). Samples in lanes 1–3 were incubated for 3 min, 30 min, and 300 min (5 h), respectively. (Section II) Plot of the densitometric analysis of the gel in Section I showing the conversion of MER-45-kDa hGH into 22-kDa hGH in the absence and presence of EDTA/EGTA at 95°C as a function of time. Concentrations of EDTA and EGTA in the incubation mixture were 0 mM (□), 1 mM (▵), and 10 mM (○).