Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
The percentage of total protein converted to 22-kDa hGH from MER-45-kDa hGH samples after a 5-h incubation in either TCEP-HCl, glutathione, or DTT at 25°C, 60°C or 95°C
| Temperature | |||
| Reductant | 25°C % 22-kDa hGH | 60°C % 22-kDa hGH | 95°C % 22-kDa hGH |
| TCEP-HCl (10 mM) | 6.0% | 20.0% | 76.0% |
| Glutathione (10 mM) | 5.0% | 27.0% | 50.0% |
| DTT (100 mM) | — | — | 55.0% |
