Table 1.
Results of the ICAT alkylation
| Peptide | Measured mass of ICAT peptides | Calculated mass of ICAT peptides | Alkylation.Identified cysteines (Fraction 1) | Reduction and alkylation.Identified cysteines (Fraction 2) |
| 72–97 | n.d. | 3465.90 | n.d. | n.d. |
| 98–104 | 1331.6 | 1331.6 | C100 | C100 |
| 180–192 | 1971.7 | 1971.9 | C187 | — |
| 216–220 | 1050.8 | 1050.2 | C219 | — |
| 246–260 | 2094.8 | 2094.5 | C257 | — |
Fraction 1: alkylation of the peptides mixture obtained from digestion of the nonreduced wt calbindin. Fraction 2: alkylation of the previous peptides mixture after reduction.