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. 2005 Apr;14(4):856–861. doi: 10.1110/ps.041185805

Figure 3.

Figure 3.

(A) Model of the nitroxide labeled M2TM using the SSNMR structure collected in DMPC (PDB code 1NYJ). The distance shown is for the lateral pair of helices, highlighted in orange. Diagonal helices would have a separation of ~25 Å (data not shown). (B) Computer models of M2TM calculated at various tilt angles demonstrate a monotonic increase in helix-axis separation with increasing tilt. The Y-axis of the figure represents changes in helix-axis separation relative to the separation at 15° tilt. Helix separation distances are calculated from helix-axis to helix-axis by averaging the coordinates of Cα for residues 22–27 at the N terminus. A 10 Å slab of each model is shown, centered on His37/Trp41, indicating the general structure of the gating interaction is intact for all tilt angles shown.