Figure 2.
Sequence of α-KTx12.1 with the sequential and medium-range NOE pattern at pH 4.5 (top) and pH 6.0 (bottom) taken from 150-msec NOESY spectra (35°C). For sequential connectivities, the thickness of the bars indicates NOE intensities; medium-range NOEs are identified by lines connecting the two coupled residues. The secondary structure was obtained from the global result of a PROCHECK-NMR (Laskowski et al. 1996) analysis of the final ensemble of NMR conformers at pH 4.5. Amino acids whose backbone amide proton resonances were not assigned at pH 6.0 are set in boxes.