Figure 2.

Fluorophore insertion sites in MglB. (A) MglB structure, in which cyan spheres indicate the sites (position relative to first codon in mature MglB is given) at which ECFP was inserted. N and C termini are labeled. (B) MglB structure with Citrine attached to the C terminus, indicated by a yellow sphere, and two permissive insertion sites of ECFP, marked by cyan spheres. (C) MglB structure with Citrine attached to the N terminus, indicated by a yellow sphere, and three permissive insertion sites of ECFP, marked by cyan spheres.