Figure 5.

Internal insertion of ECFP into YbeJ. (A) FLII⁸¹PE construct consisting of an N-terminal His-tag, ECFP (cyan), an N-terminal composite linker (CL-N), the mature YbeJ (red domains; numbers indicate amino acid positions relative to initiation codon), a C-terminal composite linker (CL-C), and Venus (yellow). (B) Emission spectra of FLIPE-600n in the presence of 0, 600 nM, and 100 μM glutamate (excitation 433 nm). (C) Emission spectra of FLII⁸¹PE-1μ in the presence of 0, 600 nM, and 100 μM glutamate (excitation 433 nm). (D) Glutamate titration curves of FLIPE-600n and FLII⁸¹PE-1μ.