Table 1.

Effect on ratio change of deletion from and insertion into composite linker sequences of linearly fused FLIPglu sensor

Sensor	CL-N deletiona	CL-C deletiona	Δ ratio	Δ ratio (%)
FLIPglu-600μ	0	0	−0.25	−8
FLIPglu-600μΔ1	0	5	−0.25	−8
FLIPglu-600μΔ2	3	2	−0.26	−8
FLIPglu-600μΔ3	10	2	−0.26	−8
FLIPglu-600μΔ4	10	10	−0.27	−8
FLIPglu-600μΔ5	13	10	−0.15	−4
FLIPglu-600μΔ5	14	10	−0.27	−10
FLIPglu-600μΔ7	15	10	−0.12	−4
FLIPglu-600μΔ8	13	13	−0.5	−20
FLIPglu-600μΔ9	14	13	−0.25	−9
FLIPglu-600μΔ10	15	13	−0.45	−15
FLIPglu-600μΔ11	13	16	−0.6	−23
FLIPglu-600μΔ12	14	16	−0.5	−19
FLIPglu-600μΔ13	15	16	−0.7	−28
	CL-N insertiona	CL-C insertiona	Δ ratio	Δ ratio (%)
FLIPglu-600μLI1	6	0	−0.25	−8
FLIPglu-600μLI2	127	0	0	0

^a Number of amino acids deleted from, or inserted into, N- and C-terminal composite linker sequences, respectively.