Figure 3.

Inhibitory effects of B. maxima albumin on trypsin. (A) Trypsin (30 nM) was incubated with various amounts of BmA-skin (▵), BmA-serum (▪) or BSA (▴) for 15 min at 25°C. The residual amidolytic activity of trypsin was determined. (B) Time stability of the inhibition of B. maxima albumin on trypsin activity. Trypsin (30 nM) was incubated with the buffer (♦), 45 nM of BmA-skin (▵), BmA-serum (▪), or BSA (▴) at 25°C for various times and then the residual amidolytic activity of trypsin was measured.