Table 1.
Free energy measurements at different temperatures for proline variants of the Notch ankyrin domain
| ΔG°u (kcal mol−1) | |||||
| Variant | 287.15 K | 289.15 K | 291.15 K | 293.15 K | 295.15 K |
| PA1 | 8.17 ± 0.09 | 8.11 ± 0.09 | 7.82 ± 0.06 | 7.82 ± 0.06 | 7.34 ± 0.03 |
| PA3 | 7.76 ± 0.06 | 7.62 ± 0.03 | 7.32 ± 0.10 | 6.92 ± 0.03 | 6.60 ± 0.02 |
| PA4 | 7.75 ± 0.06 | 7.49 ± 0.09 | 7.25 ± 0.06 | 6.90 ± 0.04 | 6.60 ± 0.09 |
| PA6 | 7.04 ± 0.03 | 6.80 ± 0.05 | 6.56 ± 0.01 | 6.34 ± 0.03 | 5.96 ± 0.03 |
| PA7 | 6.52 ± 0.05 | 6.30 ± 0.04 | 6.06 ± 0.05 | 5.83 ± 0.04 | 5.43 ± 0.02 |
| SP2 | 4.78 ± 0.04 | 4.66 ± 0.01 | 4.42 ± 0.03 | 4.08 ± 0.03 | 3.82 ± 0.02 |
| SP2AP5 | 8.38 ± 0.03 | 8.18 ± 0.04 | 7.91 ± 0.02 | 7.62 ± 0.03 | 7.25 ± 0.02 |
| PA134 | 6.95 ± 0.06 | 6.76 ± 0.03 | 6.46 ± 0.07 | 6.12 ± 0.03 | 5.73 ± 0.06 |
| PA1346 | 6.22 ± 0.01 | 5.94 ± 0.02 | 5.60 ± 0.03 | 5.18 ± 0.03 | 4.72 ± 0.03 |
| PA13467 | 4.15 ± 0.09 | 3.93 ± 0.09 | 3.61 ± 0.05 | 3.15 ± 0.10 | 2.74 ± 0.10 |
| Nankl-7* | 8.24 ± 0.05 | 8.09 ± 0.07 | 7.82 ± 0.05 | 7.52 ± 0.04 | 7.16 ± 0.02 |
The uncertainties represent standard error on the mean. Conditions: 150 mM NaCl, 25 mM Tris-HCl at pH 8.0.