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. 2005 Sep;14(9):2387–2404. doi: 10.1110/ps.041324305

Figure 5.

Figure 5.

Thermal denaturation followed by protein fluorescence, ANS fluorescence, and anisotropy at different pHs. (A) Thermal unfolding traces followed by fluorescence at selected pHs for 200 μM of CA-C: pH 4 (open circles), pH 6 (white squares), and pH 9 (filled squares). (B) The thermal denaturation experiments followed by the emission intensity at 520 nm of 20 μM CA-C and 100 μM of ANS at several pHs: pH 4 (filled squares), pH 7 (open circles), and pH 11 (filled circles). The scale on the Y-axis for both fluorescence measurements is arbitrary. (C) The change in anisotropy at 20 μM (filled squares) and 200 μM of CA-C (blank squares) at pH 7. The lines are the fittings to Equation 6, taking into account that the free energy is given by Equation 7.