Figure 2.

Amino acid alignment of the class II hydrophobins of Trichoderma/Hypocrea used in this study. The aa sequences were trimmed to show only the area from the first to the eight cysteine. Absolutely conserved aa's are within a black background, and functionally conserved aa's highlighted in grey. The symbols and letters over the alignment show the position of the four beta-strands (S1–S4) and the single helix (indicated by a horizontal cylinder). The sequence below the alignment proposes an updated consensus sequence for the Trichoderma/Hypocrea class II HFBs, as derived from this study: therein, the cysteines are in red and numbered in order of their appearance in the sequence; X denotes any amino acid, and the subscript the number of them; "al" denotes any aliphatic, hydrophobic amino acid (A, V, L, I,)