Table 2.

Suppression by A23187 and TG of VCR- and Dox-induced apoptosis in p53-nonexpressing M1-neo cells and its inhibition by CsA

Addition	% cell viability
	VCR			Dox
	None	+ CsA	+ Ver (5 μM)	None	+ CsA	+ Ver* (1 μM)
None	30.6  ± 6.9	21.3  ± 4.2	20.6  ± 3.5	44.0  ± 3.3	25.5  ± 1.1	21.1  ± 3.6
IL-6	75.0  ± 5.8	65.9  ± 4.6	63.8  ± 5.2	65.1  ± 5.3	44.6  ± 2.5	30.8  ± 3.1
A23187	67.8  ± 4.8	23.5  ± 3.3	65.2  ± 4.9	70.1  ± 3.5	22.1  ± 3.3	43.1  ± 5.1
TG	67.0  ± 3.1	31.3  ± 2.9	57.3  ± 6.0	72.5  ± 4.1	27.2  ± 3.5	40.2  ± 4.8

M1-neo cells were cultured without (None) or with the apoptosis inhibitors IL-6 (10 ng/ml), A23187 (0.4 μM), or TG (10 nM). Percent cell viability was determined 26 hr after addition of 0.82 μM VCR or 1.84 μM Dox in the absence (None) or presence of 0.1 μM CsA or 1 μM or 5 μM verapamil (Ver) and the apoptosis inhibitors above. Results with 20 μM flunarizine were similar to those with 5 μM verapamil. 

^*Verapamil was reduced to 1 μM because addition of 5 μM verapamil with 1 μM Dox resulted in less than 1% viable cells.