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. Author manuscript; available in PMC: 2008 Feb 23.
Published in final edited form as: Insect Biochem Mol Biol. 2007 Nov 17;38(2):190–200. doi: 10.1016/j.ibmb.2007.10.008

Figure 3.

Figure 3

Activity assay of recombinant AaJHA15. Purified His-JHA15 fusion protein was tested for chymotrypsin activity using synthetic substrate AAPF (N-succinyl-Ala-Ala-Pro-Phe-nitroanilide). The experiment was performed as described in Materials and Methods. JHA15 was activated by trypsin treatment, and the enzyme activity is shown as the increase in the absorption at 410 nm. A blank control was used to correct the spontaneous hydrolysis of the substrate. Chymostatin is a strong inhibitor of many proteinases, including chymotrypsin, chymotrypsin-like serine proteinases, chymases, and lysosomal cysteine proteinases. Values are means ± SEM.