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. Author manuscript; available in PMC: 2008 Feb 23.
Published in final edited form as: Insect Biochem Mol Biol. 2007 Nov 17;38(2):190–200. doi: 10.1016/j.ibmb.2007.10.008

Figure 6.

Figure 6

JH enhances the JHA15 mRNA levels in the newly emerged female adults. (A) Effects of topical application of JH on the JHA15 expression. Abdominal ligations were performed on female mosquitoes within 30 minutes of adult emergence. These abdomens were then treated with 200 ng of JH III dissolved in 0.5 μl of acetone. mRNA levels of AaET and JHA15 were analyzed using semi quantitative RT-PCR. rpS7 mRNA was measured as internal control. The RT-PCR products were stained with ethidium bromide. (B) Transcription of JHA15 in response to increasing doses of JH and methoprene. Females mosquitoes were ligated within 30 minutes of adult emergence, and the abdomens were topically treated with the indicated amounts of JH III or Methoprene. The experimental doses of JH and methoprene were chosen based on a previous experiment performed by Noriega et al. (1997). The samples were collected at 6 h after hormone application, and were subjected to real time RT-PCR analysis. Representative data (mean±SEM) from at least three independent experiments are shown.