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. Author manuscript; available in PMC: 2008 Feb 23.
Published in final edited form as: Insect Biochem Mol Biol. 2007 Nov 17;38(2):190–200. doi: 10.1016/j.ibmb.2007.10.008

Figure 8.

Figure 8

Polysome distribution of the JHA15 mRNA in Aedes aegypti before and after blood feeding. Whole body extracts from non-blood-fed female mosquitoes (PE 5d) or blood-fed mosquitoes (PBM 1h) were sedimented by centrifugation in a 20−60% sucrose gradient, and 0.35-ml fractions were collected. (A) Absorbance profiles at 254 nm (top) are shown together with analysis of rRNA. RNA was extracted from each fraction and half of the RNA was used in electrophoresis on 1% agarose/formaldehyde gel followed by ethidium bromide staining. The positions of the 40S, 60S, 80S, and polysomal peaks are indicated. (B) The remaining purified RNA was used for RT-PCR analysis of the AaET and JHA15 genes. This experiment was performed three times with similar results.