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. Author manuscript; available in PMC: 2008 Feb 23.
Published in final edited form as: Insect Biochem Mol Biol. 2007 Nov 17;38(2):190–200. doi: 10.1016/j.ibmb.2007.10.008

Figure 9.

Figure 9

Effect of injection of JHA15 dsRNA on expression of the late trypsin gene. Newly emerged female mosquitoes were injected with dsRNA corresponding to either AaET or JHA15, or both. Firefly luciferase dsRNA was used as an injection control as described by Lu et al. (2006). The injected mosquitoes were allowed a period of 5 days for recovery and were then fed blood. (A) Mosquitoes were collected at the indicated time points after a blood meal. The extracted RNAs were analyzed by real time RT-PCR and normalized to the rpS7 mRNA for each sample. WT, uninjected Ae. aegypti Rockefeller/UGAL strain; Luc, injected with double-stranded RNA complementary to the firefly luciferase gene; AaET, injected with AaET dsRNA; JHA15, injected with JHA15 dsRNA; AaET+JHA15, injected with AaET and JHA15 dsRNA. AaLT, the Ae. aegypti late trypsin gene. (B) Female mosquitoes were collected at 5 days after RNA injection (before blood feeding) and the JHA15 proteins in the midgut were examined by Western blot analysis. The lower panel shows levels of β-actin as a loading control.