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. Author manuscript; available in PMC: 2008 Feb 24.
Published in final edited form as: Cell. 2007 Jul 13;130(1):141–152. doi: 10.1016/j.cell.2007.05.026

FIG. 5.

FIG. 5

Cortical diffusion constant measurements by fluorescence recovery after photobleaching. Recovery curve of bleached wild-type Drosophila embryos expressing Bcd-GFP during mitosis 13. Bleaching was done with a scanning two-photon microscope in a volume of 16 × 16 × 7 μm3 at the anterior tip of the egg. The bleaching pulse was generated by increasing the laser power 2-fold for a duration of 5 seconds. Data points are spaced 0.5 sec and shown as blue dots. Red curve represent a fit to the solution of the diffusion equation (see Methods), yielding a diffusion constant D = 0.27 ± 0.07 μm2/s.