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. Author manuscript; available in PMC: 2009 Jan 1.
Published in final edited form as: Microb Pathog. 2007 Aug 12;44(1):52–60. doi: 10.1016/j.micpath.2007.08.004

Fig. 1.

Fig. 1

Purification of PGA polysaccharides by gel filtration chromatography. (A) Elution profile of the aqueous layer of the hot-phenol extract of A. actinomycetemcomitans JK1044 biofilms on a Sephacryl S-300 column irrigated with water. Aliquots (200 μL) of each 5-mL fraction were assayed for aminosugars (●, A485) and neutral sugars (○, A530). Void (Vo) and total (Vt) volumes of the column are indicated with arrows. (B and C) Elution profiles of sonic extracts from biofilms produced by A. pleuropneumoniae strain IA1 [12] (B) and S. epidermidis strain RP62A [35] (C).