Table 3.

Role of TLR2 in the early inflammatory response in the lungs after infection with S. pneumoniae D39 or PLN.

	D39		PLN
	WT	TLR2 KO	WT	TLR2 KO
cfu	6.5 ± 1.4 × 106	5.5 ± 1.3 × 106	13.2 ± 1.3 × 106	10.6 ± 1.8 × 106
TNF-α	9 781 ± 780	4 656 ± 375**	9 124 ± 1 203	2270 ± 236**
IL-1β	5 754 ± 714	3 566 ± 348*	18 963 ± 2 522	8716 ± 1939*
IL-10	31 ± 2	32 ± 3	BD	BD
MIP-2	42 193 ± 2 529	23 855 ± 3 396***	9 114 ± 1 112	2643 ± 505**
KC	61 120 ± 2 879	23 266 ± 3 124***	16 776 ± 2 314	4783 ± 1352**
MPO	76.7 ± 9.3	41.6 ± 3.4*	15.5 ± 3.4	12.4 ± 4.0
TLIS	18.4 ± 0.5	15.1 ± 0.8*	9.5 ± 0.9	5.3 ± 1.3*

Mice were intranasally infected with 5 × 10⁷ cfu S. pneumoniae D39 or PLN, and whole lung homogenates were obtained 6 h later. Data are means ± SEM (n = 8 per group).

^*P < 0.05 versus WT mice.

^**P < 0.001 versus WT mice.

^***P < 0.01 versus WT mice.

TNF-α, IL-1β, IL-10, MIP-2 and KC values are in pg ml⁻¹; cfu values are in cfu ml⁻¹ lung; MPO levels are in μg ml⁻¹; TLIS (total lung inflammation score) in arbitrary units.

BD, below detection limit.