Table 2. Data of test samples determined to be acceptable a).
| Sample dilution | O.D. 405 | %CV |
|---|---|---|
| ≤ 1: 500 | 0.25 ≤OD< 0.3 | <70% b) |
| 0.3 ≤OD< 0.4 | <50% b) | |
| 0.4 ≤OD< 0.5 | <40% b) | |
| 0.5 ≤OD< 1.6 | <30% b) | |
| > 1: 500 | 0.25 ≤OD< 1.6 | <30% |
First quality control of standard curve was tested for each ELISA plate, then the QC of unknown sample values were tested. Any data that did not match in the category above were not reliable.
The values of %CV were decided based on the mean plus 2SD of samples in the indicated O.D. range (Fig. 6).