Figure 2.

Leukocytic infiltration and GAG release in a.i.a. knees after intraarticular injection of Ad.sIL-1-RI-Ig and Ad.sTNF-RI-Ig. a.i.a. was induced in both knees of four groups of eight rabbits. Twenty-four hours after induction groups of rabbits received injection of adenoviral vectors as indicated. (A) At 3 and 7 days after adenoviral injection, knees of rabbits were lavaged. Numbers of infiltrating leukocytes (white blood cells; WBC) in synovial fluids for both right and left knees of each group are shown as indicated. (B) GAG levels in lavage fluids were determined by 1,9-dimethylmethylene blue assay and are shown for each group as indicated. Error bars designate ± one standard deviation. To determine whether differences observed among the groups of rabbits, left and right knees, and the days were significant, the appropriate F tests were performed. For A, differences among the groups, knees, and days, P values equal 0.0001, 0.0365, and 0.0008, respectively. For B, differences among the groups, knees, and days, P values equal 0.0001, 0.0149, and 0.0312, respectively.