Figure 4.
Expression of luciferase activity in various tissues after intraarticular injection of Ad.luciferase into a.i.a. knees. Twenty-four hours after induction of a.i.a. in both knees of two rabbits, 1.5 × 109 pfu of Ad.luciferase was injected into the left knee joint (L.), and 7 × 107 pfu of Ad.lacZ was injected into the right knee joint (R.). Seven days after injection of adenovirus, the rabbits were sacrificed. Blood was collected, knees were lavaged, and synovium, heart, liver, lung, spleen, and kidney tissues were harvested. Luciferase activities in lysates from tissues and leukocytes from lavages of right (R.) and left (L.) knees and blood (peripheral [P.] leukocytes) were quantitated and are expressed relative to luciferase activity found in synovium and leukocytes from the left knee.