TABLE 2.
Antibody Information
| Antigen | Clone or Catalog | Dilution | TSA | Species | Application | Manufacturer |
|---|---|---|---|---|---|---|
| Nrf2 | H300 | 1:200 | Yes | Rb | IHC, IF | Santa Cruz Biotechnology, Santa Cruz, CA |
| Nrf2 | C20 | 1:2,000 | Yes | Rb | IHC, IF | Santa Cruz Biotechnology |
| Nrf2 | KAP-TF125 | 1:200 | Yes | Rb | IHC | Stressgen, Victoria, BC, Canada |
| Nrf2 | H300 | 1:1000 | No | Rb | IB | Santa Cruz Biotechnology |
| GFAP | Z0334 | 1:80 | No | Rb | IF | DAKO, Carpinteria, CA |
| MAP2 | SMI-52 | 1:500 | No | Ms | IF | Covance, Berkeley, CA |
| HNE | HNE11-S | 1:50 | No | Rb | IF | Alpha Diagnostics International, San Antonio, TX |
| GAPDH | MAB374 | 1:100 | No | Ms | IB | Chemicon International, Temecula, CA |
| APP, amyloid fragment | 4G8 | 1:20,000 | No | Ms | IF | Gift from Drs. Lee and Trojanowski, University of Pennsylvania |
| NFT | AT8 | 1:300 | No | Ms | IF | Pierce, Rockford, IL, |
Summary of antibodies used in this study are shown. If antibody staining was amplified with tyramide amplification system (TSA) for immunofluorescent or immunostaining, it is indicated (yes). The species of animal in which the primary antibody was raised is indicated (species; mouse—Ms, rabbit—Rb). Applications using antibodies include immunohistochemistry (IHC), immunofluorescence (IF), and immunoblotting (IB).
Nrf2, nuclear factor E2-related factor 2; GFAP, glial fibrillary acid protein; MAP2, microtubule associate protein 2; HNE, hydroxylnonenal; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; APP, amyloid precursor protein; NFT, neurofibrillary tangle.