Figure 2.

Immunoblot analysis of SR-BI protein expression in control and homozygous mutant mice. A total of 50 μg of protein from postnuclear supernatants of mSR-BI transfected CHO cells and from liver, spleen, brain, heart, testis, and kidney, and 20 μg from the adrenal gland, of representative control (+/+) and SR-BI att (−/−) mice was analyzed by gel electrophoresis and immunoblotting with a rabbit polyclonal anti-human SR-BI antibody. ∗, 2.5-fold less protein was loaded in this, relative to the other, lanes.