Figure 5.

Selective Advantage of Corrected Cells of the Erythroid Lineage

(A) Representative FACS analysis of BM cells and peripheral RBCs from each group of mice. BM cells were analyzed for Ter-119 expression, and three different groups were differentiated by their SSC and FSC profiles (upper left part): (I) RBCs, (II) intermediate normoblasts, and (III) early normoblasts. BM cells were also analyzed for Gr-1 expression (upper middle part). GFP expression was then analyzed in each population of erythroid (Ter-119) and myeloid (Gr-1) BM cells and in peripheral RBCs (right part).

(B) Ratio of the percentage of GFP expression between the different populations of erythropoietic cells and Gr-1-positive cells. ^∗p < 0.01 versus MNDpGFP vector in normal mice; ^∗∗p < 0.001 versus EFpGFP vector in normal and CEP mice; ^∗∗∗p < 0.001 versus ESpUROS-EFpGFP vector in the different populations of erythropoietic cells of CEP mice.

Values for (B) represent mean ± SD.