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. 2008 Mar 5;3(3):e1738. doi: 10.1371/journal.pone.0001738

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Hayashi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) The number of PGCs in E11.5∼E13.5 gonads and in neonatal testes. A single dot shows the number of PGCs or germ cells in a pair of gonads or testes (open circles; Dicer^F/+ TNAP-Cre, closed circles; DicerCKO). Black bar shows the mean. (B) Immunostaining for Mvh (green) in fetal and neonatal testis. The images are merged with DAPI (blue). Scale bar: 250 µm. (C) The reduced percentage of PGCs at M-phase in E12.5 DicerCKO male gonads. The percentages of PGCs immunostained for phosphorylated Ser10 of histone H3 are shown. Error bars represent standard deviation determined from three gonads.