Fig. 4. Results of various evaluation techniques.
(A) Reverse transcription-polymerase chain reaction (RT-PCR) using primers for the shorter (lanes A and B) and longer (lanes C and D) alternatively spliced gene products. Lanes A and C, B6; lanes B and D, B6.kdkd. RNA was extracted from kidneys. (B) Western blot using kidney extracts and the rat antiserum against the shorter gene product. Lane A, B6; lane B, B6.kd/kd.