Table 1.
ivi sequences that are distinct within and between Salmonella serovars
| Organism |
ivi gene probes*
|
tpi | spvB | ||||||
|---|---|---|---|---|---|---|---|---|---|
| Class I
|
Class II
|
Class III
|
|||||||
| iviVI-A | iviXVII | iviXVIII | iviXIX | iviXXI | iviXXII | iviXXIV | |||
| Salmonella | |||||||||
| S. typhimurium 14028 | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ |
| S. newport | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ |
| S. choleraesuis | ++++ | ++++ | ++++ | ++++ | ++++ | − | − | ++++ | ++++ |
| S. typhi Ty2 | ++++ | ++++ | ++++ | ++++ | + | − | − | ++++ | − |
| E. coli† | |||||||||
| EPEC | − | + | − | − | − | − | − | +++ | − |
| UPEC | − | − | − | − | − | − | − | +++ | − |
| APEC | − | − | − | − | − | − | − | +++ | − |
| RDEC | − | − | − | − | − | − | − | +++ | − |
| XL1 | − | − | − | − | − | − | − | +++ | − |
| Other pathogens | |||||||||
| Shigella flexneri 2a‡ | − | − | − | − | − | − | − | +++ | − |
| Shigella dysenteriae SHA-1 | − | − | − | − | − | − | − | +++ | − |
| Klebsiella pneumoniae‡ | − | +++ | − | − | − | − | − | +++ | − |
| Serratia marcescens‡ | − | ++ | − | − | − | − | − | +++ | − |
| Citrobacter freundii‡ | − | − | − | − | − | − | − | +++ | − |
| Pseudomonas aeruginosa 103 | − | ++ | − | − | − | − | − | ++ | − |
| Aeromonas hydrophila SSU | − | − | ++ | − | − | − | − | ++ | − |
| Enterobacter aerogenes‡ | − | − | − | − | − | − | − | ++ | − |
| Enterobacter cloacae‡ | − | − | − | − | − | − | − | ++ | − |
| Plant pathogens | |||||||||
| Pantoea agglomerans‡ | − | − | − | − | − | − | − | + | − |
| Erwinia carotovora‡ | − | − | − | − | − | − | − | + | − |
DNA prepared from ivi genes of atypical base composition (≤49% or ≥57%) were used as molecular probes to hybridize against genomic DNA isolated from a series of gram-negative pathogens. G + C content of the ivi probes was determined by sequencing 233 bp to 326 bp from the ivi join: iviVI-A, 42%; iviXVII, 63%; iviXVIII, 46%; iviXIX, 60%; iviXXI, 38%; iviXXII, 26%; iviXXIV, 57%; tpi (51% over 172 bp); spvB (49% over 127 bp).
Enteropathogenic E. coli (EPEC O127:K63), uropathogenic E. coli (UPEC O1:K1:H7), avian pathogenic E. coli (APEC O1:K1), rabbit diarrheal E. coli (RDEC-1).
ATCC type strain.
++++, a signal equal to that seen with parental S. typhimurium DNA; −, a signal less than or equal to a 10-fold dilution of parental S. typhimurium DNA; +, ++, and +++ represent signals intermediate to that seen with 10-fold diluted and full-strength parental S. typhimurium DNA.