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. 2008 Mar 5;3(3):e1731. doi: 10.1371/journal.pone.0001731

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Yu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The percentage on each CpG site is the methylation percentage of ^mC/(^mC+C) on this site. ^mC: methylated cytosine, C: unmethylated cytosine. The sequence above the peak is the sequence to assay. The x axes are the dispensation nucleotides to the sequencing reaction based on the assayed sequences. The y axes show light emission obtained as relative light units. A: ALVE region1 in blood (the mean value of methylation percentage is 81.8±2.9 in line 7₂ and 13.2±1.2 in 6₃); B: ALVE region2 in blood (the mean value of methylation percentage is 85±1.6 in line 7₂ and 47.6±2.3 in 6₃); C: ALVE region3/4 in blood (the mean value of methylation percentage is 80.8±4.8 in line 7₂ and 68.8±6.7 in 6₃); D: Promoter region of TVB in Hypothalamus (the mean value of methylation percentage is 45.7±14.8 in line 7₂ and 37.0±14.1 in 6₃).